除草剂减量施用对麦田杂草的防治效果及对叶绿素SPAD值的影响

探讨除草剂减施对麦田杂草的防治效果,以及对小麦生长过程中叶绿素SPAD值变化的影响。结果表明,药后45 d,10%苯磺隆WP 9.00 g/hm~2处理(减施60.00%)、10%苯磺隆WP 9.00 g/hm~2+5%(唑啉草酯+炔草酯)EC 30.00 g/hm~2处理(减施60.00%)、7%双氟·炔草酯OD 63.00 g/hm~2处理(减施25.00%)和28%唑草·苯磺隆WP 16.80 g/hm~2处理(减施33.33%)对麦田阔叶杂草的鲜质量防效分别为98.64%、98.99%、98.54%和98.68%,表明除草剂减量施用对麦田杂草仍具有较好的防效。施用除草剂后,小麦叶片叶绿素SPAD值呈现先下降再上升的趋势。药后3 d、5 d、7 d,28%唑草·苯磺隆WP 33.60 g/hm~2处理(增施33.33%)小麦叶片叶绿素SPAD值显著低于7%双氟·炔草酯OD 63.00 g/hm~2处理(减施25.00%)、10%苯磺隆WP 9.00 g/hm~2处理(减施60.00%)和空白对照(CK)的叶绿素SPAD值,低于其他处理的叶绿素SPAD值。     

Dietary α‐Ketoglutarate supplementation alleviates harmful effects of high environmental ammonia on grass carp,Ctenopharyngodon idella

Current intensive fish farming usually causes high environmental ammonia (HEA) in ponds that is toxic to fish. α‐Ketoglutarate (α‐KG) can be rapidly transaminated to glutamic acid and further aminated to glutamine. Therefore, we hypothesized that dietary α‐KG supplementation would alleviate HEA toxicity to fish. To test the hypothesis, 270 healthy grass carp (Ctenopharyngodon idella) juveniles were randomly assigned to control, HEA (18.37 mg/L ammonia) and HEA + α‐KG (0.75% of α‐KG) groups. Ammonia and free amino acid content in plasma and brain, liver glutamic pyruvic transaminase and glutamic oxaloacetic transaminase activity, and urea and glycogen content were measured on the first, seventh and 42nd days. Our results showed short‐term HEA exposure (1 day) led to a significant ammonia accumulation in the brain and plasma and significantly decreased glutamic and aspartic acid content in the brain and increased glutamine content in the brain and plasma. The long‐term HEA exposure (42 days) caused significant reductions in glycine and arginine content in the brain tissue. In most cases, dietary α‐KG supplementation alleviated the fluctuations in FAA content in the brain and plasma. Our results suggested dietary α‐KG alleviated HEA toxicity to grass carp.     

面向养殖水体分布差异的COD光谱法检测研究

面向养殖水体，传统光谱法对化学需氧量（Chemical Oxygen Demand，COD）检测模型构建的基础：源域（现有样本库）与目标域（检测地水体）间光谱数据独立同分布。但是当源域与目标域分布间存在差异时，由源域得到的低误差模型常在目标域上表现下滑。针对该问题，提出面向UV Vis光谱的域对抗训练网络（DAUVwpNet），将分布不同的源域和目标域数据映射至相同分布的特征空间中，使其在该空间的分布距离尽可能接近，从而在特征空间中对源域训练的目标函数也可以迁移至目标域上，以降低模型在目标域的误差。试验表明：面向同一批测试数据，DAUVwpNet的预测误差为0.78，要低于传统模型的预测误差（0.85）；DAUVwpNet预测值与实测值间相关系数为0.95，要高于传统模型的相关系数（0.89）。表明了该网络能够较好对齐两域特征空间数据分布，降低因分布差异带来的COD检测误差。     

高温干旱复合胁迫对花生幼苗生理指标的影响

为探讨高温和干旱复合胁迫下的花生幼苗对逆境的响应机制，以抗逆性存在差异的花生品种豫花9719和豫花9326为试验材料，对其幼苗进行昼夜温度为45℃/25℃、土壤含水量为35%~40%左右的高温和干旱复合胁迫处理，研究复合胁迫对花生幼苗生长及生理特性的影响。结果表明：胁迫条件下，豫花9719的株高和干重分别比对照组低44.3%和30.2%，差异达极显著水平；含水量、叶绿素a、叶绿素b、类胡萝卜素、花青素的含量均低于对照，其中叶绿素a的含量低4.88%，差异达显著水平，花青素含量低18.14%，差异达极显著水平；PRI、PI、Gs、Pn值分别比其对照组低12%、50.31%、75.2%、72.3%，差异均达极显著水平；MDA含量和相对电导率分别比对照组高93.1%和29.93%，差异均达显著水平；可溶性蛋白和类黄酮含量分别比对照组高104.5%和41.00%，差异均达显著水平；SOD和CAT活性分别比对照组低0.91%和3.29%，差异不显著。豫花9326的株高比其对照组低33.6%，差异达极显著水平，干重降幅不显著；含水量、类胡萝卜素、花青素的含量均比对照组低，但差异均不显著；叶绿素a和叶绿素b的含量分别比对照组高19.44%和3.39%，其中叶绿素a差异达极显著水平； PRI、PI、Gs、Pn值均比对照低，差异均不显著；MDA含量和相对电导率均比对照组高，但差异均不显著；可溶性蛋白和类黄酮含量分别比对照组高271.33%和48.96%，差异均达极显著水平；SOD和CAT活性分别比对照组高70.11%和68.8%，增幅均达显著水平。推测豫花9326对高温和干旱复合胁迫的抗性较高，主要与其较为强大的抗氧化防御系统及光合系统有关。     

插装阀阀芯优化与仿真分析

插装阀在静止状态下，由于静摩擦的原因，导致阀芯与阀套出现卡滞现象；在运动状态下，由于压差和阀芯微偏移的原因造成阀芯与阀套发生磨损；为此提出一种新型的带有导流槽的插装阀阀芯。基于缝隙流动和液压卡紧分析，建立插装阀阀芯与阀套间隙的CFD优化仿真模型，通过N-S方程、伯努利方程和卡紧力方程联立得到阀芯与阀套间卡紧力的推导公式。基于CFD仿真模拟分析，比较新型阀芯与原阀芯不同模型间隙的切线应力、压力分布规律，结果表明：在入口压力为12 MPa时，原阀芯的切线应力在12 000 Pa上下波动，大于新型阀芯切线应力4 200 Pa；在入口压力为8 MPa时，原阀芯的切线应力在7 200 Pa上下波动，大于新型阀芯切线应力3 000 Pa；且原阀芯切线应力的波动范围远大于新型阀芯。新型阀芯在阀套间的受力更加平稳，磨损更小。研究结果为插装阀优化以及减少能量损失和改善润滑条件提供理论指导和依据。     

油菜高亚麻酸育种探析

为促进通过提高油菜种子亚麻酸含量途径增加中国人群亚麻酸的摄入量,提高健康水平的工作进程。结合文献资料,在剖析开展油菜高亚麻酸育种的意义、可行性、难点及可利用的有利条件的基础上,提出油菜高亚麻酸育种策略与技术途径。在工作实践中,陕西省杂交油菜研究中心研究团队创建并完善"油菜高亚麻酸种质的筛选方法",利用该方法开展油菜高亚麻酸种质资源筛选和高亚麻酸育种取得良好成效:油菜种质资源的整体亚麻酸含量、具有较高亚麻酸含量种质的百分比率、亚麻酸含量最高值、亚麻酸含量15%的种质数量均明显增加;获得一批亚麻酸含量大于15%(最高含量达21%)的甘蓝型油菜非转基因种质,育成高亚麻酸的不育系和恢复系;甘蓝型油菜高亚麻酸杂交种‘秦杂油7号’通过国家品种登记;2个亚麻酸含量15%左右的杂交种正在申请品种登记。发现在A02、C02及A06染色体上存在多个与亚麻酸显著关联SNP位点。提出开展基础机理研究和分子设计育种创新体系建设,开展高亚麻酸种质资源创新、综合评价和利用研究,构建高亚麻酸育种体系,规化高效保优栽培技术路线,开展油菜籽加工技术、抗氧化研究,构建标准体系等6方面建议。     

‘珍珠蜜’网纹甜瓜优质栽培技术探讨

‘珍珠蜜’网纹甜瓜是当前我国的高档优质果品,以其果大肉厚、耐贮运及独特的风味,深受人们喜爱。近年来,在北京、上海等大中城市十分畅销,产值极高。开展合理栽培对提高‘珍珠蜜’网纹甜瓜的产量、改善品质、降低成本、提高市场竞争力、促进主产区的经济发展、增加农民收入等具有十分重要的意义。     

中国野桑蚕人工饲料育研究初报

为解决野桑蚕作为试验昆虫在室内饲养难的问题,以江苏启东野桑蚕为材料,用家蚕低成本饲料进行了饲育研究。在催青期温度25℃、饲育温度27~25℃以及光照条件1~2龄12 L 12 D、3~5龄15 L 9 D的条件下,野桑蚕人工饲料育的2龄起蚕率为31.3%,幼虫期20~48 d,雌、雄蛹体质量分别为0.45、0.25 g,蛹期17~23 d,雌蛾平均产卵266粒,产滞育卵的母蛾占45.0%。研究结果表明,人工饲料育能够使野桑蚕顺利完成世代发育。     

杀线虫苏云金芽胞杆菌NBIN-863高含量悬浮剂的研制

目前我国微生物农药原药生产技术已达到甚至超过发达国家水平,但制剂技术却存在较大差距,主要体现在剂型结构不合理、制剂质量差、农药助剂种类及品种少、剂型加工工艺总体水平还比较落后等方面。苏云金芽胞杆菌（Bt）等功能微生物产品的市场容量及应用效果与剂型是密切相关的。为了明确新型生物杀线剂NBIN-863的最佳施用剂型,特开展此试验。通过对防腐剂、分散剂、悬浮剂等不同助剂的选择,研制了一种高含量Bt悬浮剂（5.2×10⁹～7.9×10⁹CFU/mL）。配方优化试验表明,在Bt发酵液中加入0.4%防腐剂山梨酸钾、1.0%分散剂硅酸镁铝、4.0%悬浮剂木质素磺酸钙,用盐酸调节pH至4.5,54℃热储前芽胞数最高为7.9×10⁹ CFU/mL,对秀丽隐杆线虫活性最高,LC₅₀为2.83 mg/L,对黄瓜根结线虫田间防效达68.64%。综合各指标,尤其是对秀丽隐杆线虫的半致死浓度,杀线虫NBIN-863悬浮剂（7.9×10⁹ CFU/mL）效果最好,不仅流动性好,而且耐贮藏,是防治作物根结线虫病的理想药剂。     

Being exposed to low concentrations of pirimiphos-methyl and chlorfenapyr has detrimental effects on the mobility of Trogoderma granarium

BACKGROUND

Sublethal effects of insecticides may negatively affect several biological and behavioral traits of insects. The lethal effects of pirimiphos-methyl and chlorfenapyr have been previously showed on Trogoderma granarium, but little knowledge is available about their sublethal effects at low concentrations on both sexes. Herein, the sublethal effects of pirimiphos-methyl and chlorfenapyr on the mobility of T. granarium males and females were investigated.

RESULTS

Lethal concentration (LC) values of pirimiphos-methyl and chlorfenapyr were lower for T. granarium females than males. LC values on males were LC₁₀ = 0.000788 and 0.00139 mg active ingredient (a.i.) cm⁻², LC₃₀ = 0.00350 and 0.00535 mg a.i. cm⁻², and LC₅₀ = 0.00986 and 0.0136 mg a.i. cm⁻² for pirimiphos-methyl and chlorfenapyr respectively. LC on females were LC₁₀ = 0.000704 and 0.00110 mg a.i. cm⁻², LC₃₀ = 0.00323 and 0.00428 mg a.i. cm⁻², and LC₅₀ = 0.00925 and 0.0110 mg a.i. cm⁻² for pirimiphos-methyl and chlorfenapyr respectively. The walking duration of beetles exposed to LC₃₀ of pirimiphos-methyl was significantly lower than the individuals exposed to LC₁₀ and LC₃₀ of both insecticides and control ones. Pirimiphos-methyl LC₃₀-exposed males remained more time on their back (101.7 s) than females (46.9 s), while the latter stayed immobile longer than males (381.7 s versus 371.9 s). The highest speed was recorded for control beetles (14.17 mm s⁻¹ females vs. 12.44 mm s⁻¹ males), while the lowest speed was observed in pirimiphos-methyl LC₃₀-treated males (8.36 mm s⁻¹) and females (9.66 mm s⁻¹).

CONCLUSIONS

Overall, males and females exposed to low concentrations of pirimiphos-methyl and chlorfenapyr showed reduced motility. This knowledge can be exploited further to unlock behavioral effects of insecticides for effective pest management programs in warehouses. © 2023 Society of Chemical Industry.